Generation of a murine single chain Fv (scFv) antibody specific for cucumber mosaic virus (CMV) using a phage display library.

摘要: With the long-term goal of generating CMV-resistant transgenic plants using antibody genes, a single-chain variable fragment (scFv) that binds to cucumber mosaic virus was isolated from scFv phage display library by four rounds affinity selection with CMV-Mf as an antigen. The has identical binding specificity CMV monoclonal is generated hybridoma fusion technique, and recognized purified preparations isolates belonging either subgroup I or II in immunoblotting. nucleotide sequences recombinant showed heavy chain region (V(H)) gene belonged VH3 kappa light (V kappa) came Vkappa4 subgroup. Our results demonstrate library, alternative approach traditional potential applicability study plant pathology.