Fifty years with bile acids and steroids in health and disease.

摘要: Cholesterol and its metabolites, e.g., steroid hormones bile acids, constitute a class of compounds great biological importance. Their chemistry, biochemistry, regulation in the body have been intensely studied for more than two centuries. The author has aspects biochemistry clinical chemistry steroids acids 50 years, this paper, which is an extended version Schroepfer Medal Award lecture, reviews discusses part work. Development application analytical methods based on chromatography mass spectrometry (MS) central many projects, aiming at detailed characterization quantification metabolic profiles under different conditions. In present terminology, much work may be termed steroidomics cholanoidomics. Topics discussed are human feces, acid production, bacterial dehydroxylation during enterohepatic circulation, sulfates plasma humans other primates, development neutral ion-exchanging lipophilic derivatives Sephadex sample preparation group separation conjugates, urine conditions, hydroxylation liver disease, effects alcohol-induced redox changes synthesis metabolism, biosynthesis, compartmentation with 3H-labeled ethanol, formation metabolism sulfated metabolites progesterone pregnancy, abnormal patterns these patients intrahepatic cholestasis pregnancy corrected by ursodeoxycholic acid, inherited acquired defects biosynthesis their treatment, conjugation N-acetylglucosamine, sulfate-glucuronide double conjugates hydroxycholesterols, extrahepatic 7alpha-hydroxylation 3-dehydrogenation C27 acids. final analysis free brain tissue capillary liquid chromatography-electrospray MS suggests need reevaluation function rat brain.