Induction of an effective anti-tumor immune response and tumor regression by combined administration of IL-18 and Apoptin.

摘要: Immunization strategies using plasmid DNA can potentially improve humoral and cellular immune responses that protect against cancer infectious diseases. The chicken anemia virus-derived Apoptin protein exhibits remarkable specificity in its ability to induce apoptosis tumor cells, but not normal diploid cells. Interleukin-18 (IL-18) is a Th1-type cytokine has demonstrated potential as biological adjuvant murine models. In this study, we analyzed the anti-tumor mechanism of action simultaneous IL-18 gene transfer C57BL/6 mice bearing Lewis lung carcinoma (LLC). Here report growth established tumors immunized with pAPOPTIN conjunction pIL-18 was significantly inhibited compared empty vector (EV) or alone. Furthermore, immunization elicited strong natural killer activity LLC tumor-specific cytotoxic T lymphocyte (CTL) vitro. addition, cells from lymph nodes vaccinated + secreted high levels Th1 IL-2 IFN-gamma, indicating regression related dominant response. These results demonstrate vaccination together may be novel powerful strategy for immunotherapy.