Evolution of a reverse transcriptase to map N 1 -methyladenosine in human messenger RNA
作者： Huiqing Zhou , Simone Rauch , Qing Dai , Xiaolong Cui , Zijie Zhang
DOI: 10.1038/S41592-019-0550-4
关键词:
摘要: Chemical modifications to messenger RNA are increasingly recognized as a critical regulatory layer in the flow of genetic information, but quantitative tools to monitor RNA modifications in a whole-transcriptome and site-specific manner are lacking. Here we describe a versatile platform for directed evolution that rapidly selects for reverse transcriptases that install mutations at sites of a given type of RNA modification during reverse transcription, allowing for site-specific identification of the modification. To develop …
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