Qualitative analysis and enantiospecific determination of angular-type pyranocoumarins in Peucedani Radix using achiral and chiral liquid chromatography coupled with tandem mass spectrometry.

摘要: Angular-type pyranocoumarins (APs), the derivatives of khellactone, are widely documented as main active constituents in Peucedani Radix (Chinese name: Qian-hu). Owing to natural occurrence chiral centers, enantiomers APs extensively distributed original plant, and enantioselective performances have been definitely demonstrated for these enantiomers. In current study, chemical characterization major minor was performed using ultra high performance liquid chromatography coupled with diode array detector hybrid ion trap-orbitrap mass spectrometry. On other hand, a heart-cut two-dimensional achiral–chiral combining triple quadropole-linear trap spectrometry system (2D LC–MS/MS) developed simultaneous enantiospecific quantification eighteen coumarins, including seven pairs Eleven (1–11) were recruited propose UV absorption characteristics electrospray ionization fragmentation patterns APs. A total 42 components categorized into based on their spectral properties identified according proposed pathways, while two linear-type furanocoumarins (12–13) unambiguously assigned by further purification. Capcell core RP-C18 column employed primary LC dimension achieve efficient racemic separation (1–9 12–13) Radix, Chiralpak AD-RH utilized secondary contribute enantiomerically enriched (1, 3 5–9). Collectively, results provided evidences revealing material basis therapeutic effects 2D LC–MS/MS present study is expected be an ideal tool quality control well reliable technique complex matrices containing both achiral components.