Characterization of beta papillomavirus E4 expression in tumours from Epidermodysplasia Verruciformis patients and in experimental models

摘要: This study provides a first characterisation of β-HPV life-cycle events in tumours abscised from EV patients (the human model β-HPV-induced skin cancer), and shows how changes E4 expression patterns relate to disease severity. has also been reconstructed organotypic raft cultures created using EV-derived keratinocytes. In lesions cultures, abundant cytoplasmic was detectable differentiating cells along with viral genome amplification as reported for other HPV types. seen PCNA-positive basal some cancers well HPV8CER (Complete Early Region) transgenic mice. these lesions, staining extended throughout the full thickness epithelium apparent markedly atypical cells. The loss such at tumour border suggests distinct type dysregulation that may be exploited marker during β-HPV-associated cancer progression.