Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole

摘要: Captive flatfishes, such as the Senegalese sole, typically produce very low volumes of sperm. This situation is particularly prevalent in first generation (F1) reared sole males, which limits development artificial fertilization methods and implementation selective breeding programs. In this study, we investigated whether combined treatments with homologous recombinant follicle-stimulating (rFsh) luteinizing (rLh) hormones, produced a mammalian host system, could stimulate spermatogenesis enhance sperm production F1 males. an initial autumn/winter experiment, weekly intramuscular injections increasing doses rFsh over 9 weeks resulted stimulation gonad weight, androgen release, germ cell proliferation entry into meiosis, expression different spermatogenesis-related genes, whereas subsequent single rLh injection potentiated spermatozoa differentiation. second late winter/spring trial corresponding to sole's natural prespawning spawning periods, tested effect repeated on amount quality by males previously treated for 4, 6, 8 or 10 weeks. These latter results showed that combination increase up 7 times, slightly improve motility spermatozoa, although high variability response was found. However, sustained administration during markedly diminished Leydig survival steroidogenic potential testis. data suggest vivo application effective at stimulating setting basis future establishment gonadotropin-based hormone therapies ameliorate reproductive dysfunctions species.