Comparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes

摘要: Abstract This work evaluates the activity of a few key enzymes involved in combating reactive oxygen species (ROS), such as ascorbate peroxidase (EC 1.11.1.11), catalase 1.11.1.6), glutathione reductase 1.6.4.2), and superoxide dismutase 1.15.1.1), well concentration malondialdehyde hydrogen peroxide transgenic non-transgenic soybean leaves. Additionally, differential protein from leaves both genotypes were evaluated by applying regulation factor ≥ 1.8 to further corroborate hypothesis that genetic modification itself can be stress for these plants. For this task, plants obtained seeds modified with cp4EPSPS gene. The results revealed higher activities all than (ranging 13.8 70.1%), concentrations leaves, clearly indicating condition oxidative established genotype. 47 proteins differentially abundant when comparing plants, 26 accurately identified, including (CP4EPSPS). From results, it is possible conclude plant searching new equilibrium maintain its metabolism because being maintained within levels tolerated plant. Biological significance present paper first one literature where are shown translational aspects involving cp4 EPSPS main biological importance make demystification modification, allowing answers some questions still remain unknown, enlarge our knowledge about genetically organisms. article part Special Issue entitled: Translational Plant Proteomics.