摘要: Conservation programs are challenged with preventing biodiversity loss; losses that have typically been managed as preservation of individual species and groups. An increasing focus on different levels has promoted methods evaluating the genetic diversity in these a tool loss. Therefore, most conservation practitioners today realize need to evaluate, preserve, manage population-level range-wide an essential component species. Recent methodological (e.g. non-lethal tissue sampling) technological advances high-throughput sequencing) greatly increased our ability evaluate endangered threatened butterflies. In this chapter, we provide framework guide for conducting research including it management plans Lepidoptera. We discuss variety molecular markers tools available researchers well guidance implementing them. step-by-step sampling, statistical concerns, advice troubleshooting tips. review several case studies successfully used data incorporated results into plans. The chapter concludes summary techniques, future directions, lessons learned from genetics study Lepidoptera concern.