Benzophenone-ATP: a photoaffinity label for the active site of ATPases.

摘要: Publisher Summary This chapter describes the benzophenone-ATP that is used as a photoaffinity label for active site of ATPases. The outlines methods, arguments in support use benzophenone preferred photoactive moiety design substrate analogs, referring specifically to 3'- O -(4-benzoyl)benzoyl-ATP (benzophenone-ATP or BzATP). One most attractive aspects BzATP probe, addition unique photochemical properties benzophenone, relative ease with which it synthesized. These factors should help encourage (and other “Bz-ligand” derivatives) being well suited studies ATP-utilizing enzymes. reviews underlying logic pertaining benzophenone-derivatized ligands receptor domains on diverse biological macromolecules an aqueous reaction medium. Thus, introduction new probe into arsenal useful molecular tools employed by biochemist must await its successful application laboratories.