Respiration: comparison of the Winkler technique, O 2 electrodes, O 2 optodes and the respiratory electron transport system assay

摘要: Aerobic respiration is a biological energy generation process that consumes organic carbon and oxygen. In the ocean, balance between photosynthesis recognized as critical to understanding ocean’s impact on hydrospheric atmospheric CO2. Techniques determine can be based inorganic chemistry, electrochemistry, photochemistry, enzymology. Here, for method comparison, physiological was simultaneously measured by Winkler (W), O2 electrodes (E), optodes (O). These techniques detected respiratory consumption (R), in situ, dark incubation chambers. Respiratory electron transport system activity measurements potential (Ф), biochemically. Leptomysis lingvura, marine mysid, Ulva rigida, species of green algal sea lettuce, were two organisms tested. Physiological results from each technique not statistically significantly different (multiple paired Student’s t tests, p value > 0.05) inside range similar published measurements. The mean dry-mass-specific L. lingvura U. rigida 0.147 ± 0.037 0.023 ± 0.008 µmol O2 h−1 (mg dry mass)−1, n = 9, respectively. R-to-Ф ratios organisms. However, linear regression R Ф stronger (r 2 = 0.814 0.313) than biomass 2 = 0.643 0.213). application Passing–Bablok analysis evidenced high correlation results, Bland–Altman examined average difference (“bias”) limits agreement methods.