Description of the nucleolar activity and karyotype in germinative cell lines of Rhodnius domesticus (Triatominae, Heteroptera)

作者: Alessandra Morielle , Maria Tercília Vilela De Azeredo-Oliveira

DOI: 10.1080/00087114.2004.10589369

关键词:

摘要: Abstract - The purpose of this work was to study the karyotype, spermatogenesis and nucleolar activity at meiosis, in species Rhodnius domesticus (Heteroptera, Triatominae). testicular tubules were cytologically prepared by conventional method cell crushing subsequent application cytogenetic staining techniques with lacto-acetic orcein silver-ion impregnation. under presented karyotype 2n = 20A+XY, modal number subfamily Triatominae. chromosomes no primary constriction therefore characterized as holocentric. It observed that sex sometimes located periphery, close ring formed autosomes, first meiotic division. At metaphases II, positioned center autosomal ring, thus evidencing a postreductional behavior. These same showed late migration anaphases clearly impregnated silver-ions, suggesting they bore Nucleolar Organizer Reg...

参考文章(20)
Mtv deAzeredoOliveira, M. G. Tavares, Cytogenetic studies on holocentric chromosomes of five species of triatomines (Heteroptera: Reduviidae) Cytobios. ,vol. 89, pp. 51- 62 ,(1997)
Herman Lent, Pedro W. Wygodzinsky, Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bulletin of the American Museum of Natural History. ,vol. 163, pp. 123- 520 ,(1979)
Kaori Kato, Cytochemistry and fine structure of elimination chromatin in Dytiscidae Experimental Cell Research. ,vol. 52, pp. 507- 522 ,(1968) , 10.1016/0014-4827(68)90492-8
Franz Wachtler, André Stahl, The nucleolus: A structural and functional interpretation Micron. ,vol. 24, pp. 473- 505 ,(1993) , 10.1016/0968-4328(93)90026-W
E. S. De Vaio, B. Grucci, A. M. Castagnino, M. E. Franca, M. E. Martínez, Meiotic differences between three species (Hemiptera, Reduviidae) Genetica. ,vol. 67, pp. 185- 191 ,(1985) , 10.1007/BF02424489
Jos� M. Gonz�ez-Garc�a, Julio S. Rufas, Carmen Antonio, Jos� A. Suja, Nucleolar cycle and localization of NORs in early embryos of Parascaris univalens Chromosoma. ,vol. 104, pp. 287- 297 ,(1995) , 10.1007/BF00352260
M.N. Gruzova, Z.P. Zaichikova, I.I. Sokolov, Functional organization of the nucleus in the oogenesis of chrysopa perla L. (insecta, neuroptera). Chromosoma. ,vol. 37, pp. 353- 385 ,(1972) , 10.1007/BF00284886
Norihiro Ueshima, Cytotaxonomy of the triatominae (Reduviidae: Hemiptera) Chromosoma. ,vol. 18, pp. 97- 122 ,(1966) , 10.1007/BF00326447