Evaluation of Neo-Osteogenesis in Eosinophilic Chronic Rhinosinusitis Using a Nasal Polyp Murine Model.

摘要: PURPOSE Osteitis refers to the development of new bone formation and remodeling in chronic rhinosinusitis (CRS) patients; it is typically associated with eosinophilia, nasal polyps (NPs), recalcitrant CRS. However, roles ossification CRS or without NPs remain unclear due lack appropriate animal models. Thus, necessary have a suitable model for greater advances understanding pathogenesis. METHODS BALB/c mice were administered ovalbumin (OVA) staphylococcal enterotoxin B (SEB). The numbers osteoclasts osteoblasts bony changes assessed. Micro computed tomography (micro-CT) scans conducted measure thickness. Immunofluorescence, immunohistochemistry, quantitative polymerase chain reaction performed evaluate runt-related transcription factor 2 (RUNX2), osteonectin, interleukin (IL)-13, RUNX2 downstream gene expression. Gene set enrichment analysis was mucosal tissues from control patients. effect resveratrol evaluated terms osteogenesis murine eosinophilic NP model. RESULTS histopathologic showed markedly thickened bones significant increase osteoblast OVA/SEB-treated compared phosphate-buffered saline-treated mice. structural on micro-CT consistent histopathological features. expression IL-13 increased by administration OVA/SEB positive correlation. mainly co-localized osteoblasts. Bioinformatic using human transcriptome revealed that IL-13-induced via RUNX2. Treatment resveratrol, candidate drug against osteitis, diminished RUNX2, number CONCLUSIONS In present study, we found radiographic evidence previously established This could provide insights into pathophysiology neo-osteogenesis basis developing therapeutics.