Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host

摘要: Abstract We have recently described a genetic system, termed in vivo expression technology (IVET), that uses an animal as selective medium to identify genes pathogenic bacteria specifically express when infecting host tissues. Here, the potential utility of IVET approach has been expanded with development transcriptional-fusion vector, pIVET8, which antibiotics resistance basis for selection pIVET8 contains promoterless chloramphenicol acetyltransferase (cat) and lacZY genes. A pool Salmonella typhimurium clones carrying random cat-lac transcriptional fusions, produced was used infect BALB/c mice were subsequently treated intraperitoneal injections chloramphenicol. Strains survived by expressing cat gene then screened those had low-level on laboratory medium. These strains carry operon fusions are induced (ivi genes). One ivi identified (fadB) encodes enzyme involved fatty acid oxidation, suggesting this might contribute metabolism bactericidal or proinflammatory acids. The pIVET8-based system also S. cultured macrophages. nature products will provide more complete understanding metabolic, physiological, factors virulence microbial pathogens.