GC-MS/MS detects potential pregabalin abuse in susceptible subjects' hair.

摘要: Pregabalin, a GABA analogue, binds to the alpha 2 delta subunit of voltage-dependent calcium channels. It is recognised as efficacious in pathologies such epilepsy, neuropathic pain, and anxiety disorders. Since pregabalin prescriptions have increased worldwide, reports its abuse been accumulating, mainly patients with opioid The present study investigated potential by means hair analysis, matrix that provides valuable retrospective information. Half pool 280 susceptible had occasional drug users were being monitored for driving licence renewals. other 140 history opiate dependency assess compliance methadone therapy. In view determining samples, it was extracted methanol, successfully derivatised give ethyl chloroformate derivative, finally analysed gas chromatography-tandem mass spectrometry. Selectivity, linearity, limit detection, quantification, recovery, intra- inter-day precision, accuracy quantification procedure appraised. Pregabalin limits detection 30 pg/mg 50 pg/mg, respectively. We found 10.7% samples from 4.29% positive without medical prescription. mean concentration higher than consumers indications (1.45 ng/mg vs 0.74 ng/mg). These results suggest possesses significant particularly among individuals attending dependence services serious emerging issue, which should be carefully monitored.