Cancer specific promoter CpG Islands hypermethylation of HOP homeobox (HOPX) gene and its potential tumor suppressive role in pancreatic carcinogenesis

摘要: We have recently identified HOP hoemobox (HOPX) as a tumor suppressor gene candidate, characterized by tumor-specific promoter DNA hypermethylation in human cancers, and it can remarkably inhibit tumors’ aggressive phenotypes. In this current study, we for the first time examined methylation level of HOPX tested functional relevance pancreatic cancer (PC). Clinical features was investigated 89 PC tissues, immunohistochemistry added. also its phenotype assays such soft agar, proliferation, invasion, cell cycle analysis. tissues had compared to corresponding normal pancreas uniqueness robust discriminate from (AUC = 0.85, P < 0.0001). Unexpectedly, increased expression revealed predominant Langerhans islet cells, where reduced cells with hypermethylation. transfectants exhibited G1 arrest subG1 accumulation, inhibited forming invasive ability. Defective which is consistent may explain cancer, intense turn uniquely contribute carcinogenesis.