Bovine lactoferrin decreases histopathological changes in the liver and regulates cytokine production by splenocytes of obstructive jaundiced rats.

摘要: Objective and design: The aim of this study was to evaluate effects bovine lactoferrin (BLF) on histopathological changes in the liver 14 day obstructive jaundiced (OJ) rats production tumor necrosis factor (TNF-α) interleukin 6 (IL-6) by splenocytes from 7- 14-day OJ rats. Material subjects: In 50 male Buffalo strain (170-270 g, mean 230 g) were used. Treatment: Rats given 10 mg BLF 0.5 ml saline daily, using a stomach tube. applied 2 days before operation for 13 following operation. Methods: specimens prepared standard techniques. Sections, 5 mm thick stained with hematoxylin eosin reviewed histologically. Microscopic estimation semiquantitive 4-grade scale used evaluation changes. For cytokine measurement splenocyte cultures stimulated μg/ml lipopolysaccharide (LPS). After overnight incubation activities TNFα IL-6 determined bioassays. statistical data nonparametric Mann-Whitney test applied. Results: OJ, treated BLF, pathological markedly reduced, particular foci disseminated lymphocytes, cellular necrobiosis, bile duct proliferation dilation. Neither fibrous reticular connective tissue nor activation Kupffer cells revealed. 7-day treatment caused significant inhibition both spontaneous (mean 253, median 275, vs 160, 148 pg/ml, p=0.002) LPS-induced TNF-α 4967, 4102, 2291, 2234 pg/ ml, p = 0.004) cultures. 120, 81, 43, 26 p=0.005) as well 422, 378, 293, 0.025) also lowered. On other hand, upregulated production, 148, 158, 338, 196, 0.001) (m ean 1331, 1507, 2239, 1707 0.027). Conclusion: We conclude that oral administration is beneficial alleviating deleterious OJ.