Adaptation of Skyline for Targeted Lipidomics.

摘要: In response to the urgent need for analysis software that is capable of handling data from targeted high-throughput lipidomics experiments, we here present a systematic workflow straightforward method design and selected reaction monitoring in based on lipid building blocks. Skyline powerful primarily designed proteomics applications where it widely used. We adapted this tool “Plug Play” system research. This extension offers unique capability assemble mass spectrometry methods complex lipids easily by making use With simple yet tailored modifications, analyze main classes such as glycerophospholipids, sphingolipids, glycerolipids, cholesteryl-esters, cholesterol can be quickly introduced into easy application end users without distinct bioinformatics skills. To illustrate benefits our novel strategy, used quantify sphingolipi...