Identification of differentially regulated genes of Plasmodium by suppression subtractive hybridization.

摘要: Plasmodium, the causative agent of malaria, has many morphologically and functionally distinct developmental stages. In mosquito host alone, there are five transitions during development a gametocyte into sporozoite. Determining which genes expressed at different stages is vital to our understanding parasite. There growing number techniques designed study gene expression, including microarray. Here, Johannes Dessens, Gabrielle Margos, Maria del Carmen Rodriguez Robert Sinden describe novel method: suppression subtractive hybridization (SSH) its successful application in obtaining midgut stage-specific Plasmodium.