Structure of the human gene for α‐enolase

摘要: In mammals there are at least three isoforms of the glycolytic enzyme enolase encoded by similar genes: α, β and γ. this report we describe isolation characterization human α-enolase locus. The gene appears to exist as a single copy in haploid genome is composed 12 exons distributed over more than 18000 bases. structure has high degree similarity that rat γ-enolase genes, with identical positions for all intron regions. Primer extension S1 nuclease protection experiments indicate transcription initiated multiple sites. putative promoter region, like other housekeeping lacks canonical TATA CAAT boxes, extremely G + C-rich contains several potential SP1 binding Furthermore, various sequences known regulatory elements were detected.