Expression of Escherichia coli folylpolyglutamate synthetase in the Chinese hamster ovary cell mitochondrion.
作者： B.F. Lin , B. Shane
DOI: 10.1016/S0021-9258(17)36940-5
关键词:
摘要: Chinese hamster ovary (CHO) cell transfectants expressing Escherichia coli folylpoly-gamma-glutamate synthetase (FPGS) activity solely in their cytosol lack mitochondrial folylpolyglutamates and are auxotrophic for glycine. Addition of a mammalian leader sequence targeted E. FPGS to the mitochondria these cells. Mitochondrial expression restored folylpolyglutamate pools overcame glycine requirement. Pteroyltriglutamates functioned as effectively longer glutamate chain length folates found wild type CHO cells metabolic cycle synthesis provided they were located mitochondria. Although folypolyglutamates cannot enter mitochondria, can be released without prior hydrolysis possessed normal cytosolic pools. The proportion cellular folate mitochondrion is governed by competition between activities.
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