Mutants of the cruciferous plant Arabidopsis thaliana lacking glycine decarboxylase activity.

摘要: A mutant of Arabidopsis thaliana (L.) Heyn. (a small plant in the crucifer family) that lacks glycine decarboxylase activity owing to a recessive nuclear mutation has been isolated on basis growth requirement for high concentrations atmospheric CO2. Mitochondria from leaves did not exhibit glycine-dependent O2 consumption, release 14CO2 [14C]glycine, and catalyse glycine-bicarbonate exchange reaction is considered be first partial associated with cleavage. Photosynthesis was decreased after illumination under conditions promote partitioning carbon into intermediates photorespiratory pathway, but impaired non-photorespiratory conditions. Thus required any essential function unrelated photorespiration. The photosynthetic response probably caused by an increased rate glyoxylate oxidation, which results sequestering all readily transferable amino groups metabolically inactive pool, depletion photosynthesis cycle. exogenously applied [14C]glycollate 14-fold lower than wild type, suggesting decarboxylation only significant source