Membrane fluidity of Toxoplasma gondii: a fluorescence polarization study
作者： Yves Gallois , Françoise Foussard , Andrée Girault , Jocelyne Hodbert , Anne Tricaud
DOI: 10.1111/J.1768-322X.1988.TB00701.X
关键词:
摘要: Toxoplasma gondii membrane fluidity was investigated by fluorescence polarization. We used 1,6-diphenyl 1,3,5-hexatriene (DPH) as a fluorescent hydrophobic probe. Fluorescence anisotropy (r) and degree of order (s) showed high properties. Chemical analysis performed on this parasite. found low cholesterol/phospholipid ratio, many unsaturated fatty acid chains, phosphatidylcholine sphingomyelin amounts. These results were in good agreement with the observed fluidity. This may be related to great adaptability infesting wide variety host cells.
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