Properties of β-glucosidase purified from Aspergillus niger
作者： H. H. Yeoh , T. K. Tan , S. L. Chua , G. Lim
DOI: 10.1007/BF00940168
关键词:
摘要: Extracellular β-glucosidase fromAspergillus niger USDB 0355 was purified 120-fold. It gave a single band on PAGE and had an Mr of 325 000. optimally active at 60°C pH 4.6. Km values forp-nitrophenyl-β-glucoside cellobiose 0.82±0.10mm 1.33±0.20mm, respectively. competitively inhibited by glucose non-competitively (mixed) glucono-δ-lactone.
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