Arginase I expression is upregulated by dietary restriction in the liver of mice as a function of age

摘要: Arginase is a cytosolic enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. This reaction comprises final step urea cycle, which provides principal route for disposal nitrogenous waste from protein catabolism. The present study investigates normal endogenous activity expression level arginase I as function age in liver 2-, 6-, 18-month-old mice. effect dietary restriction (DR) on was also investigated two groups mice, 2- 18-month old. assessed spectrophotometrically, further confirmed by Western blotting analyses. mRNA measured using real-time PCR. Our results show (U/mg protein) higher 2-month-old mice decreased gradually with age. In contrast, observed be older compared younger DR seen upregulate both findings concluded down-regulated advancement upregulated DR. suggests plays an important role maintaining related metabolic processes