Characterization of cis elements that regulate the expression of glnA in Synechococcus sp. strain PCC 7942.

摘要: The upstream noncoding region of the Synechococcus sp. strain PCC 7942 (hereafter referred to as 7942) glnA gene was fused cat in order study expression both and Escherichia coli. lack E. coli indicated that promoter not recognized by RNA polymerase. construct integrated into chromosome at a neutral site. Expression reporter regulated under various nitrogen conditions way similar gene. A deletion introduced binding site NtcA regulatory protein abolished derepression during growth nitrate starvation. Deletion sequence between transcription translation start sites prevented repression observed ammonium. These results indicate is subject complex regulation involves sequences downstream from