Rapid ocular angiogenic control via naked DNA delivery to cornea.
作者： Anthony P. Adamis , Anthony P. Adamis , Yasufumi Moromizato , Yasufumi Moromizato , Robert J. D’Amato
DOI:
关键词:
摘要: PURPOSE To determine the efficacy and safety of naked plasmid gene therapy to corneal stroma epithelium. METHODS Naked DNA was injected under pressure into cornea mice. The expression genes coding for beta galactosidase (beta-gal), enhanced green fluorescent protein (EGFP), vascular endothelial growth factor (VEGF), soluble Flt-1 (s-Flt) recorded measured with regard dose, time course, bioactivity. RESULTS LacZ beta-gal demonstrated as early 1 hour, persisting 10 days. Plasmid-injected corneas remained clear free inflammation. EGFP bicistronically expressed VEGF demonstrate practicality simultaneous in vivo analysis Corneal injection a containing cDNA induced anterior chamber neovascularization. Moreover, form receptor effectively prevented CONCLUSIONS is readily accessible laboratory clinic. method described safe, effective, titratable, easily monitored. delivery has potential alter treatment wide variety segment diseases.
arvojournals.org参考文章(13)
D.L. Tanelian, M.A. Barry, S.A. Johnston, T. Le, G. Smith, Controlled Gene Gun Delivery and Expression of DNA Within the Cornea BioTechniques. ,vol. 23, pp. 484- 488 ,(1997) , 10.2144/97233ST06
R J D'Amato, J Folkman, E Flynn, C C Chen, B M Kenyon, E E Voest, A model of angiogenesis in the mouse cornea. Investigative Ophthalmology & Visual Science. ,vol. 37, pp. 1625- 1632 ,(1996)
E. E. Voest, B. M. Kenyon, M. S. O'Reilly, G. Truitt, R. J. D'Amato, J. Folkman, Inhibition of Angiogenesis In Vivo by Interleukin 12 JNCI Journal of the National Cancer Institute. ,vol. 87, pp. 581- 586 ,(1995) , 10.1093/JNCI/87.8.581
Jeffrey M Isner, Arterial gene transfer of naked DNA for therapeutic angiogenesis: early clinical results. Advanced Drug Delivery Reviews. ,vol. 30, pp. 185- 197 ,(1998) , 10.1016/S0169-409X(97)00115-4
J. Wolff, R. Malone, P Williams, W Chong, G Acsadi, A Jani, P. Felgner, Direct gene transfer into mouse muscle in vivo. Science. ,vol. 247, pp. 1465- 1468 ,(1990) , 10.1126/SCIENCE.1690918
Vladimir Budker, Tatayana Budker, Guofeng Zhang, Vladimir Subbotin, Aaron Loomis, Jon A. Wolff, Hypothesis: naked plasmid DNA is taken up by cells in vivo by a receptor-mediated process. Journal of Gene Medicine. ,vol. 2, pp. 76- 88 ,(2000) , 10.1002/(SICI)1521-2254(200003/04)2:2<76::AID-JGM97>3.0.CO;2-4
Heiko E. Von Der Leyen, Rudiger Braun-Dullaeus, Michael J. Mann, Lunan Zhang, Joseph Niebauer, Victor J. Dzau, A pressure-mediated nonviral method for efficient arterial gene and oligonucleotide transfer. Human Gene Therapy. ,vol. 10, pp. 2355- 2364 ,(1999) , 10.1089/10430349950017004
Stephen L. Hart, Carolina V. Arancibia-Cárcamo, Margreet A. Wolfert, Carolina Mailhos, Nicola J. O'reilly, Robin R. Ali, Charles Coutelle, Andrew J. T. George, Richard P. Harbottle, Andrew M. Knight, D. Frank P. Larkin, Roland J. Levinsky, Leonard W. Seymour, Adrian J. Thrasher, Christine Kinnon, Lipid-Mediated Enhancement of Transfection by a Nonviral Integrin-Targeting Vector Human Gene Therapy. ,vol. 9, pp. 575- 585 ,(1998) , 10.1089/HUM.1998.9.4-575
Douglas W. Losordo, Peter R. Vale, James F. Symes, Cheryl H. Dunnington, Darryl D. Esakof, Michael Maysky, Alan B. Ashare, Kishor Lathi, Jeffrey M. Isner, Gene Therapy for Myocardial Angiogenesis Circulation. ,vol. 98, pp. 2800- 2804 ,(1998) , 10.1161/01.CIR.98.25.2800
KAZUO TSUBOTA, HIROKO INOUE, KIYOSHI ANDO, MASAFUMI ONO, KENICHI YOSHINO, ICHIRO SAITO, Adenovirus-mediated gene transfer to the ocular surface epithelium. Experimental Eye Research. ,vol. 67, pp. 531- 538 ,(1998) , 10.1006/EXER.1998.0557