Method for producing web protein, a fused protein, recombinant dna, an expression vector, a host cell and strain-producers
作者： Konstantin Vasilevich Sidoruk , Mikhail Yurievich Beburov , Irek Ilyasovich Gubaydullin , Vladimir Grigorievich Bogush , Vladimir Georgievich Debabov
DOI:
关键词:
摘要: The invention relates to the field of biotechnology and provides a method for producing recombinant proteins from orb-weaving spider silk in yeast cells. This involves construction an expression vector which comprises DNA sequence encoding protein fused with ubiquitin-like occupying N-terminal position respect within protein. hybrid gene makes it possible increase tens times production protein, wherein accumulates cells water-insoluble fraction form processed free component. also comprising sequences proteins, DNAs host vectors suitable carrying out method, producer strains silk.
lens.org 参考文章(32)
Philip J. Barr, Ubiquitin expression system ,(1995)
M.B. Hinman, R.V. Lewis, Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. Journal of Biological Chemistry. ,vol. 267, pp. 19320- 19324 ,(1992) , 10.1016/S0021-9258(18)41777-2
Dagmara Motriuk, John E. Gatesy, Cheryl Y. Hayashi, Randolph V. Lewis, Spider silk protein encoding nucleic acids, polypeptides, antibodies and method of use thereof ,(2002)
Tadas Panavas, Jeffrey G. Marblestone, Raymond J. Peroutka, Tauseef R. Butt, Amolkumar Karwa, Methods and compositions for enhanced protein expression and purification ,(2004)
Daniel Huemmerich, Christian Ackerschott, Thomas Scheibel, Recombinant spider silk proteins ,(2005)
Michael B. Hinman, Randolph V. Lewis, Ming Xu, Spider silk protein ,(1995)
S. R. Fahnestock, S. L. Irwin, Synthetic spider dragline silk proteins and their production in Escherichia coli. Applied Microbiology and Biotechnology. ,vol. 47, pp. 23- 32 ,(1997) , 10.1007/S002530050883
Stefan Müller, Michael J Matunis, Anne Dejean, Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus The EMBO Journal. ,vol. 17, pp. 61- 70 ,(1998) , 10.1093/EMBOJ/17.1.61
E. S. Johnson, The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer The EMBO Journal. ,vol. 16, pp. 5509- 5519 ,(1997) , 10.1093/EMBOJ/16.18.5509
S. Arcidiacono, C. Mello, D. Kaplan, S. Cheley, H. Bayley, Purification and Characterization of Recombinant Spider Silk Expressed in Escherichia Coli Applied Microbiology and Biotechnology. ,vol. 49, pp. 31- 38 ,(1998) , 10.1007/S002530051133