Recombinational DNA double-strand breaks in mice precede synapsis
作者： Shantha K. Mahadevaiah , James M.A. Turner , Frédéric Baudat , Emmy P. Rogakou , Peter de Boer
DOI: 10.1038/85830
关键词:
摘要: In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for phosphorylated histone (gamma-H2AX, which marks the sites of DSBs) to investigate timing, distribution and Spo11-dependence DSBs in mouse. We show that, as yeast, mouse form during leptotene. Loss gamma-H2AX staining (which irradiated somatic cells temporally linked with DSB repair) spatially correlated synapsis, even when this synapsis 'non-homologous'.
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