PREPARATION OF IMMOBILIZED TANNINS FOR PROTEIN ADSORPTION
作者： Ichiro Chibata , Ryujiro Sano , Takao Mori , Taizo Watanabe , Tetsuya Tosa
DOI: 10.1007/BF02991844
关键词:
摘要: Preparation and adsorption specificity of tannins immobilized by covalent binding on water-insoluble matrices were investigated. Immobilized prepared condensing cyanogen bromide activated with aminohexyl derivatives several kinds matrices. The most suitable matrix for the immobilization tannin was alkali-treated cellulose powder. concentration sodium hydroxide solution alkali treatment influenced capacity a protein, but temperature time did not. having spacers long chain length exhibited high protein. Chinese gallotannin favorable ligand protein adsorption. also possible using epichlorohydrin instead bromide. maximum about 50 mg/ml absorbent. adsorbed proteins specifically not absorb low molecular weight compounds.
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