Production of TNF-alpha, IL-6 and TGF-beta, and expression of receptors for TNF-alpha and IL-6, during murine Mycobacterium avium infection.

摘要: The Mycobacterium avium complex comprises intracellular bacteria associated with disseminated infection in patients acquired immune deficiency syndrome (AIDS). Immune defects that lead to are unknown but cytokines appear play an important role the immunomodulation of host defence mechanisms. We evaluated cytokine profiles seen temporally after murine M. infection. Spleen cells were obtained from avium-infected C57BL/6 mice and uninfected at weeks 1, 2, 3, 4 5. Cells cultured vitro subsequently pulsed killed avium. Supernatants collected splenic concentrations interleukin-6 (IL-6), transforming growth factor-beta 1 (TGF-beta 1) tumour necrosis factor-alpha (TNF-alpha) measured. TGF-beta was detected week followed by IL-6 production 2. Elevated TNF-alpha levels observed 3. addition polyclonal anti-TGF-beta antibody peritoneal macrophages presence cell supernatants 3 5 led decreased bacterial counts compared controls. Anti-IL-6 did not have any effect on macrophage anti-mycobacterial activity. Concurrently, we expression receptors infected macrophages. propose early elevated a known suppressor function, conjunction down-regulation may help explain suboptimal response TNF-alpha, leading impaired