Cationic extracts of egg yolk and their effects on motility, survival and fertilising ability of bull sperm

摘要: Abstract Cationic extracts of egg yolk were obtained by sequential extraction and precipitation with amaranth. The three obtained, which water soluble (WTM), saline (SLM) citrate (CTM), cationic at pH 7.6 varied in solubility ionic strength. WTM extract was superior to SLM CTM 5% sustaining survival bull sperm 37°C protecting against cold shock. Lipid protein levels lipid:protein ratio highest WTM. Increasing concentrations improved increased protection A field trial as a diluent for ambient temperature proved that it maintained fertilizing ability better than control during Days 1–6 after collection sperm. fraction may bind firmly the plasma membrane because its charged nature also compete detrimental seminal peptides binding membrane.