Timing and location of zona pellucida synthesis during oogenesis in domestic cats--an ultrastructural immunohistological investigation.

摘要: The application of zona pellucida proteins for contraception wildlife and feral animals, including stray cats, has been promoted since it was demonstrated to be effective free-roaming mares. Active immunization with leads either reversible or irreversible infertility. Therefore, knowledge the timing location synthesis during oogenesis in cats is a key aspect designing an immunocontraceptive felids. Domestic cat ovaries obtained after ovariohysterectomy were used produce specific rabbit antibody against feline zonae pellucidae. Ultrathin sections (70 nm) treated anti-zona followed by incubation gold-labelled anti-rabbit IgG ultrastructural investigation. gold label related ultrastructure oocytes granulosa cells. Cat follicles at different stages development examined. reacted very specifically fully grown compact pellucida. In secondary follicles, labels found on inside cells vicinity oocytes. Primary labelled their cubic fragments cleft between oocyte. Some primordial characterized labelling conclusion, these results indicate that produced exclusively not Synthesis takes place every stage follicular development.