CREB-Binding protein acetylates hematopoietic transcription factor GATA-1 at functionally important sites.

摘要: The transcription factor GATA-1 is a key regulator of erythroid-cell differentiation and survival. We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to zinc finger domain GATA-1, markedly stimulates activity required for erythroid differentiation. Here we report CBP, but not p/CAF, acetylates at two highly conserved lysine-rich motifs present C-terminal tails both fingers. Using [3H]acetate labelling experiments anti-acetyl lysine immunoprecipitations, show acetylated in vivo same sites by CBP vitro. In addition, acetylation an E1A-sensitive manner, thus establishing correlation between GATA-1. Acetylation vitro did alter ability bind DNA, mutations either motif affect DNA binding expressed mammalian cells. Since certain functions are revealed only environment, constructs were examined their trigger terminal when introduced into GATA-1-deficient cell line. found partially impaired induce while abrogated it completely. Taken together, these data indicate important suggest novel mechanism which regulates