Device Engineering for Infectious Disease Diagnosis using Isothermal DNA Amplification and Lateral Flow Detection

摘要: of the Dissertation Device Engineering for Infectious Disease Diagnosis using Isothermal DNA Amplification and Lateral Flow Detection By Kristina Roskos Keck Graduate Institute Applied Life Sciences: 2013 Technologies that enable infectious diseases diagnosis in low-resource settings could greatly facilitate effective treatment containment such diseases. Nucleic acid amplification testing can be used to identify pathogens, but typically requires highlytrained personnel large, expensive lab equipment, neither which is available settings. Our overall goal develop a portable diagnostic system utilizes low-cost, disposable, mesofluidic cartridge handheld electronics unit perform fully-integrated nucleic at point care As first step toward this goal, we developed subunit execute isothermal coupled with lateral flow detection, parallel development sample preparation by our collaborators Claremont BioSolutions. Fluid handling inside detection facilitated through one-way passive valves, flexible pouches, electrolysis-driven pumps, promotes compact inexpensive instrument design. The closed-system disposable prevents workspace amplicon contamination. design based on standard, scalable manufacturing techniques, as injection molding. Using an initial prototype system, demonstrated purified Mycobacterium tuberculosis genomic DNA. We then refined conjunction improved instrument, automates pumping, heating, timing, format compatible eventual integration subunit. This incorporates novel, inexpensive, stand-alone, valve, smaller, integrated pump components, more complex molded polycarbonate core piece, enhanced chambers improve visual detection. independent valve component tailored variety fluidic systems. appropriate thermal control, successful within have separate module master-mix reagent storage reconstitution designed act interface between upstream envision merger these two subunits into will user-friendly, automated sample-in answer-out primary countries high disease burden.