Stress Response in Dairy Cows Related to Rectal Examination

摘要: Background: Currently, rectal palpation is the cheapest and most convenient method which using for detecting of internal diseases, gynecologic conditions artifi cial inseminations in dairy cows. However, procedure probably painful, resulting physiological behavioral stress reactions. The life metabolic activity can be affected by oxidative one stress-related negative outcomes characterized accumulation radical oxygen species. Many factors such as stress, diet, antibiotics husbandry practices effect animals’ health growth performance. aim study was emphasized to effects related response oxidant /antioxidant status Materials, Methods & Results: conducted on ten 3-4 years old, non-pregnant Holstein performed a closed area traditional method. Blood samples were taken from each animal, an hour before just after white blood cell, red hemoglobin levels, hematocrit value, total antioxidant status, nitric oxide, glucose cholesterol well plasma cortisol concentrations. WBC, RBC, Hct Hb values measured whole automatic count device. Plasma TAS TOS levels determined commercially available kit. Nitric oxide decomposes rapidly aerated solutions form stable nitrite/nitrate products. nitrite/ nitrate concentration modifi ed Griess assay. concentrations ELISA Glucose analyzed serum auto-analyzer. White cell signifi cantly increased compared baseline (P < 0.05) whereas no cant difference found Serum have decreased palpation, markedly 0.05), relevant variation 13.41% 23.26% respectively. Moreover, remarkable increase circulating 0.05 factor 31.78%) observed palpation. In parallel, also factor: 13.51%) On other hand, depressed intensity remained weak (variation -7.24%). Discussion: present results indicate that will considered stressful condition, evidenced strong increases cortisol, NOx associated with decreases addition, could additional responsible amplifi cation. A more comprehensive identifi cation changes during benefi further researches terms accurate management cattle industry. determination parameters provide novel approaches evaluation cattle.