Structural determinants of the capacity of heparin to inhibit the proliferation of vascular smooth muscle cells

摘要: Previous work from our laboratory has demonstrated that both anticoagulant and nonanticoagulant heparin species can inhibit the proliferation of vascular smooth muscle cells in vivo vitro. In this communication, we report studies on structure-function relationships to its antiproliferative effect cells. These were carried out by preparing discrete sizes fragments chemically modifying heparin. The compounds tested for their ability rat calf aortic cell growth. minimum fragment size which retains some growth inhibitory activity is a hexasaccharide; maximal was obtained with dodecasaccharide larger fragments. Both O-sulfation N-substitution found be important effect. Comparison activities different allowed us identify several molecules have lost properties, but retain activity.