A comparison of cell wall properties of Arabidopsis thaliana

作者: Robert John Palin

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摘要: Mechanical properties of the plant cell wall are important in many industrial applications including biofuels, food quality and biotechnology. The consists a network cellulose microfibrils cross-linked with hemi-cellulose interpenetrated by pectin. It is known that changes composition architecture lead to detectable differences mechanical properties, but relationship not yet fully understood. In this work, three mutations Arabidopsis thaliana, ida, mur1 qua-2 (Snakeskin/sks), were compared Columbia (col0) wild type. Shoot root growth characterised evaluate effects on growth. ida mutation behaved like col0, sks showing increasingly severe Cells also grown suspension culture an investigation components both tissue cultured cells was conducted. An increase pectin caused culturing process, content due found. mean force required break (the rupture force) deformation at obtained compression testing. Force-deformation data from compressions mathematically modelled up deformations below elastic limit walls, allowing derivation low strain modulus (E). Significant reductions E for col0 between observed. Similarities drawn genotype single levels significantly different shoot, material properties.

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