Changes in the Rumen Papillae During the Periparturient Transition in Holstein Dairy Cows are Accompanied by Changes in Abundance of Proteins Involved in Intracellular pH Regulation, But not SCFA Transport

摘要: The objective of this study was to examine changes in SCFA transport capacity the rumen epithelium during parturition transition period. Twelve pregnant, cannulated primiparous and multiparous cows were fed a standard Total Mixed Ration (TMR) for duration dry period; after calving, all switched an early lactation TMR. Rumen pH, fluid biopsies taken 3 weeks before anticipated calving (PRE), 1 week actual (PERI) 6 (POST). analyzed protein abundance using immunofluorescence. Immunofluorescence analysis showed no significant monocarboxylate co-transporter, isoform among PRE, PERI POST periods (16878±1555 A.U., 15613±1508 A.U. 16886±1555 respectively; MCT1). Also, sodium/proton exchanger, (NHE3) not significantly different (10467±1066 9465±1066 8552±1109 respectively). Sodium/Bicarbonate Co-transporter, (NBC1) likewise unaffected by time, with changing from 16467±1274 13983±1318 15047±1324 respectively. Abundance Carbonic Anhydrase, 2 (CA2) decreased 12332±1580 A.U (PRE) 7235±1580 8121±1643 (POST) decrease (p = 0.01 PRE v. PERI). observed suggest that long-term adaptation occurs increase capacity. Decreased CA2 suggests intracellular acidification is occurring as part epithelial remodelling. Taken together, implies that, periparturient transition, pH leads epithelium, promoting remodelling result increased