Inhibition of glioma growth by microbubble activation in a subcutaneous model using low duty cycle ultrasound without significant heating.

摘要: Object In this study, the authors sought determine whether microbubble (MB) destruction with pulsed low duty cycle ultrasound can be used to reduce brain tumor perfusion and growth through nonthermal microvascular ablation. Methods Studies using C57BLJ6/Rag-1 mice inoculated subcutaneously C6 glioma cells were approved by institutional animal care use committee. Microbubbles injected intravenously, 1 MHz was applied varying cycles every 5 seconds for 60 minutes. During treatment, heating quantified. Following growth, hemodynamics, necrosis, apoptosis measured. Results Tumor blood flow significantly reduced immediately after posttreatment ranging from 36% (0.00002 cycle) 4% (0.01 of pretreatment flow. Seven days necrosis increased in all treatment groups, while 0.005 0.01 ...