Negative Regulation of Class IA Phosphoinositide 3-kinase by Protein Kinase Cδ Limits Fcγ Receptor-mediated Phagocytosis in Macrophages
作者： K. Hazeki , K. Inoue , K. Nigorikawa , O. Hazeki
DOI: 10.1093/JB/MVN142
关键词:
摘要: Stimulation of macrophages by various ligands results in the activation both phosphoinositide 3-kinase (PI3K) and protein kinase C (PKC). Here, we showed that PKCdelta selectively inhibits class IA PI3K. Prior exposure to a PKC activator, phorbol 12-myristate 13-acetate (PMA) inhibited PI3K induced Fcgamma receptor (FcgammaR) ligation but not C5a. Prolonged inhibition GF109203X increased basal activity quiescent macrophages. The effect inhibitor can be observed from mice lacking IB (p110gamma). Thus was suggested attenuate activity. Chronic PMA degradation Akt activation. Enhancement actvity also cells stably deficient prepared shRNA technique. FcgammaR-mediated phagocytosis dramatically these cells. it is inactivation functioning regulation phagocytosis.
oup.com
sci-hub.se 参考文章(34)
Hong Liu, Yaling Qiu, Lei Xiao, Fan Dong, Involvement of Protein Kinase Cε in the Negative Regulation of Akt Activation Stimulated by Granulocyte Colony-Stimulating Factor Journal of Immunology. ,vol. 176, pp. 2407- 2413 ,(2006) , 10.4049/JIMMUNOL.176.4.2407
Michihiro Matsumoto, Wataru Ogawa, Yasuhisa Hino, Kensuke Furukawa, Yoshitaka Ono, Mikiko Takahashi, Motoi Ohba, Toshio Kuroki, Masato Kasuga, Inhibition of Insulin-induced Activation of Akt by a Kinase-deficient Mutant of the ε Isozyme of Protein Kinase C Journal of Biological Chemistry. ,vol. 276, pp. 14400- 14406 ,(2001) , 10.1074/JBC.M011093200
H D Gresham, A Zheleznyak, J S Mormol, E J Brown, Studies on the molecular mechanisms of human neutrophil Fc receptor-mediated phagocytosis. Evidence that a distinct pathway for activation of the respiratory burst results in reactive oxygen metabolite-dependent amplification of ingestion. Journal of Biological Chemistry. ,vol. 265, pp. 7819- 7826 ,(1990) , 10.1016/S0021-9258(19)39003-9
M A Tucci, S L Newman, L K Mikus, Differential requirements for cellular cytoskeleton in human macrophage complement receptor- and Fc receptor-mediated phagocytosis. Journal of Immunology. ,vol. 146, pp. 967- 974 ,(1991)
K Hazeki, T Seya, T Okada, M Ui, N Ninomiya, O Hazeki, Y Fukui, Involvement of phosphatidylinositol 3-kinase in Fc gamma receptor signaling. Journal of Biological Chemistry. ,vol. 269, pp. 22732- 22737 ,(1994) , 10.1016/S0021-9258(17)31707-6
Ken Tsukamoto, Kaoru Hazeki, Megumi Hoshi, Kiyomi Nigorikawa, Norimitsu Inoue, Takehiko Sasaki, Osamu Hazeki, Critical Roles of the p110β Subtype of Phosphoinositide 3-Kinase in Lipopolysaccharide-Induced Akt Activation and Negative Regulation of Nitrite Production in RAW 264.7 Cells Journal of Immunology. ,vol. 180, pp. 2054- 2061 ,(2008) , 10.4049/JIMMUNOL.180.4.2054
Luowei Li, Keeran Sampat, Nancy Hu, Julia Zakari, Stuart H. Yuspa, Protein kinase C negatively regulates Akt activity and modifies UVC-induced apoptosis in mouse keratinocytes. Journal of Biological Chemistry. ,vol. 281, pp. 3237- 3243 ,(2006) , 10.1074/JBC.M512167200
Hui C Wen, Wan C Huang, Adnan Ali, James R Woodgett, Wan W Lin, Negative regulation of phosphatidylinositol 3-kinase and Akt signalling pathway by PKC. Cellular Signalling. ,vol. 15, pp. 37- 45 ,(2003) , 10.1016/S0898-6568(02)00047-5
W.S Liu, C.A Heckman, The Sevenfold Way of PKC Regulation Cellular Signalling. ,vol. 10, pp. 529- 542 ,(1998) , 10.1016/S0898-6568(98)00012-6
Jyoti SRIVASTAVA, Katarzyna J. PROCYK, Xavier ITURRIOZ, Peter J. PARKER, Phosphorylation is required for PMA- and cell-cycle-induced degradation of protein kinase Cδ Biochemical Journal. ,vol. 368, pp. 349- 355 ,(2002) , 10.1042/BJ20020737