Investigating the transient and persistent effects of heat on Clostridium difficile spores

摘要: Purpose: Clostridium difficile spores are extremely resilient to high temperatures. Sublethal temperatures associated with the ‘reactivation’ of dormant spores, and utilized maximize C. spore recovery. Spore eradication is vital importance food industry. The current study seeks elucidate transient persisting effects heating at various temperatures. Methods: Spores five strains different ribotypes (001, 015, 020, 027 078) were heated 50, 60 70–80 °C for 60 min in phosphate-buffered saline (PBS) enumerated 0, 15, 30, 45 60 min. GInaFiT was used model kinetics inactivation. In subsequent experiments, transferred enriched brain heart infusion (BHI) broths after 10 min 80 °C heat treatment PBS; samples 90 min 24 h. Results: all demonstrated log-linear inactivation tailing when [(x=7.54±0.04 log10 vs 4.72±0.09 colony-forming units (c.f.u.) ml− 1; P<0.001]. At 70 °C, except 078 exhibited substantial decline recovery over Interestingly, 50 °C had an inhibitory effect on 0 (7.61±0.06 c.f.u. 1 6.13±0.05 P<0.001). Heating 70/80 °C inhibited initial germination outgrowth both newly produced aged broths. This inhibition appeared be transient; 24 h vegetative counts higher heat-treated non-heat-treated (x=7.65±0.04 6.79±0.06 P<0.001). Conclusions: more resistant Heat initially inhibits germination, but populations accelerates period.