Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence for mordanting effect.

摘要: Gallotannin, consisting mainly of low molecular weight esters such as penta- and hexagalloylglucoses (commercially available tannic acid produced from Turkish nutgall), can be used for increasing diversifying tissue contrast in electron microscopy. When applied on specimens previously fixed by conventional methods (aldehydes OsO4), the galloylglucoses (LMGG) penetrate satisfactorily cells induce general high with fine delineation extra- intracellular structures, especially membranes. In some features, additional details their intimate configuration are revealed. Various experimental conditions tested indicate that LMGG display a complex effect tissues: they act primarily mordant between osmium-treated structures lead, concomitantly stabilize components against extraction incurred during dehydration subsequent processing. Experiments aldehyde blocking reagents (sodium borohydride glycine) suggested mordanting is not dependent residual aldehydes groups tissues.