Tyrosine nitration in plasma proteins from patients undergoing hemodialysis

摘要: ● Background: A growing body of evidence suggesting that oxidative stress might be one the most important complications occurring during hemodialysis (HD) has accumulated. However, although role reactive oxygen species been investigated extensively, little is known about involvement nitrogen species. In present investigation, levels protein modifications in form tyrosine nitration patients undergoing long-term HD therapy were evaluated. Methods: Tyrosine plasma proteins was detected by means Western blotting using a specific nitrotyrosine-recognizing monoclonal antibody, and band intensity evaluated image analysis software. Immunoprecipitation antinitrotyrosine-agarose‐ conjugated antibodies, followed blotting, used an attempt to identify nitrated proteins. Results: Although several with residues observed healthy individuals, increased some all tested (n 25) compared controls 6). At least 6 apparent bands appeared more than their counterparts from controls. Ceruloplasmin identified as 1 significantly patients. Conclusion: Results investigation show are posttranslationally modified residues. The nature these proteins, well exact molecular mechanisms consequences modifications, warrant additional investigation. Am J Kidney Dis 44:286-292. © 2004 National Foundation, Inc.