Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

摘要: In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill (FG) cells its teratogenicity zebrafish embryos. The FG was with methods including MTT neutral red uptaking (NRU), lactate dehydrogenase (LDH) releasing Hoechst 33342 propidium idodide (PI) double staining. Moderate in a concentration-dependent manner observed. 24 h-IC50 value 53.48 ± 1.21, 59.13 1.19 46.21 1.24 mg L−1 obtained through MTT, NRU LDH assays, respectively. Double fluorescence staining demonstrated that induced death mainly necrosis. There no significant found exposed highest testing concentration (20 L−1, P > 0.05) as by Comet assay. Zebrafish embryos at concentrations ≥10 displayed moderate toxic effects on survival, spontaneous movement, hatching, heart rates their development, which were evidenced yolk pericardial sac edemas, body length reduction tail flexure time- manners specific stages. h-, 48 h- 96 h-LC50 values 41.80 1.10, 17.80 1.04 14.46 1.05 These results suggested is moderately cultured vitro embryos, similar sensitivity LC50 indicated.