NUCKS overexpression in breast cancer

摘要: Background NUCKS (Nuclear, Casein Kinase and Cyclin-dependent Substrate) is a nuclear, DNA-binding highly phosphorylated protein. A number of reports show that NUCKS expressed on the level mRNA in several human cancers, including breast cancer. In this work, expression both RNA protein levels was studied tissue biopsies consisted invasive carcinomas, intraductal proliferative lesions, benign epithelial proliferations fibroadenomas, as well primary cultures derived from above biopsies. Specifically, order to evaluate correlation with histopathological features disease, immunohistochemistry employed paraffin sections types. addition, by means Reverse Transcription PCR (RT-PCR), real-time (qRT-PCR) Western immunoblot analyses cell developed same