Novel aspects of tomato root colonization and infection by Fusarium oxysporum f. sp. radicis-lycopersici revealed by confocal laser scanning microscopic analysis using the green fluorescent protein as a marker

摘要: The fungus Fusarium oxysporum f. sp. radicis-lycopersici is the causal agent of tomato foot and root rot disease. green fluorescent protein (GFP) was used to mark this in order visualize analyze colonization infection processes vivo. Transformation F very efficient gfp expression stable for at least nine subcultures. Microscopic analysis transformants revealed homogeneity signal, which clearly visible hyphae as well chlamydospores conidia. To our knowledge, first report shown. transformation did not affect pathogenicity. Using confocal laser scanning microscopy, colonization, infection, disease development on roots were visualized detail several new aspects these observed, such (i) complete pattern system; (ii) steps contact between host, takes place hair zone by mingling attachment hairs; (iii) preferential sites surface, are grooves along junctions epidermal cells; (iv) absence specific sites, emergence secondary roots, tips, or wounded tissue, structures, appressoria. results work prove that use GFP a marker F. convenient, fast, effective approach studying plant-fungus interactions.