Identification of E74-like factor 1 (ELF1) as a transcriptional regulator of the Hox cofactor MEIS1

摘要: Objective Myeloid ectropic viral integration site 1 ( MEIS1 ) is a Hox cofactor known for its role in development and strongly linked to normal leukemic hematopoiesis. Although previous studies have focused on identifying protein partners of transcriptionally regulated targets, little about the upstream transcriptional regulators this tightly gene. Understanding regulation important understanding hematopoiesis leukemogenesis. Materials Methods Here we describe our focusing evolutionary conserved putative promoter region. Phylogenetic sequence analysis reporter assays MEIS1-expressing (K562) nonexpressing (HL60) cell line models were used identify key regulatory regions potential transcription factor binding sites within candidate region followed by functional expression one identified regulator both lines primary human cord blood leukemia samples. Results Chromatin status associated with expression. Truncation mutation coupled revealed that ETS family member located 289 bp annotated start required activity. Of three members tested, only ELF1 was enriched as assessed electrophoretic mobility shift assay chromatin immunoprecipitation experiments K562. This finding confirmed -expressing Moreover, small interfering RNA−mediated knockdown K562 cells decreased Conclusions We conclude an positive