GENETICS OF SOMATIC MAMMALIAN CELLS : I. DEMONSTRATION OF THE EXISTENCE OF MUTANTS WITH DIFFERENT GROWTH REQUIREMENTS IN A HUMAN CANCER CELL STRAIN (HELA).

摘要: The parental HeLa cell population, a morphologically uniform, human cancer strain, grown for several years in tissue culture by procedures always involving massive inocula, has been shown to contain different mutant types. Two clonal lines have isolated and established as reliable stock cultures. Both strains exhibit 100 per cent plating efficiency high or low serum concentrations the presence of feeder system. In absence system concentrations, two are quantitatively differentiable: S3 still exhibits efficiency, while that S1 lies neighborhood zero. These differences remained stable throughout successive generations growth each strain including 2 single isolations. Application these techniques studies genetics mammalian somatic cells specific cell-cell interactions indicated.