Effect of ciprofloxacin on microbiological development in wetland mesocosms

摘要: An understanding of how antibiotics and other "emerging contaminants" affect both water treatment systems natural environments is growing interest. Ciprofloxacin a broad-spectrum antibiotic active against Gram-positive Gram-negative bacteria has been extensively used over the past 20 years. The objective this research was to study effect an such as ciprofloxacin on development, function stability bacterial communities in wetland systems. Four mesocosm wetlands planted with Phragmites australis were initially seeded activated sludge from waste plant allowed develop for 1 week period, after which 2 4 mesocosms exposed concentration μg/mL 5 day period. then monitored several microbiological hydrological parameters course 22 weeks. community species distribution catabolic capabilities assessed via denaturing gradient gel electrophoresis (DGGE) level physiological profiling (CLPP), based carbon utilization. DGGE results indicated that decreased total number PCR-amplifiable DNA (bacteria) overall diversity operational taxonomic units (OTUs). Through CLPP it shown interstitial adversely affected by ciprofloxacin, creating temporary decrease activity inherent communities. However, 2-5 recovery period activities returned levels comparable those found not ciprofloxacin. These findings suggest exposure may have adverse reducing their ability assimilate anthropogenic carbon-based compounds; however, normal functionality resume It also observed plants ciprofloxacin-treated did adapt presence, instead showing initial browning above ground parts eventual die-off. Reduced porosity, evapotranspiration, mixing observed.